Comparing the proteomes of human mesenchymal stem cells and human osteoblasts using gene ontologies

Roman M. Salasznyk, Aaron M. Westcott, Robert F. Klees,
Donald F. Ward, Zhi Xiang, Scott Vandenberg,
Kristin P. Bennett and George E. Plopper


Stem Cells and Development, v. 14(4), August 2005.

GO Charts and Interactive Gene Ontologies: GO charts for biological process and molecular function categories, plus individual gene ontology reports for each relevant DAVID category. The reports also contain, for each protein, up-to-date links to related information in PubMed and MedLine.

Sorted Protein Lists: Master gene ontology reports, with PubMed and MedLine links where appropriate, for four kinds of proteins (classified, unclassified, unclassifiable, and unknown).

Raw Protein Lists: Raw data in two text files (hMSC, hOST).

Full Text: More complete text of paper.


The events regulating the osteogenic differentiation of human mesenchymal stem cells are not known. Two-dimensional gel electrophoresis and liquid chromatography tandem mass spectrometry were used to identify the proteomes of undifferentiated stem cells and osteoblasts at a 200 pmol threshold; gene ontology methods were used to identify and classify differences in these proteomes on the basis of cellular function. These differences suggest that changes in clusters of functionally related proteins accompany the maturation of stem cells to osteoblasts.

Corresponding author:

George E. Plopper, Ph.D.
Assistant Professor Department of Biology
Rensselaer Polytechnic Institute
110 8 Street
Troy NY 12180-3596
(518) 276-8288 phone
(518) 276-2162 fax

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This work was supported by Grant #1R01EB002197-01 from the National Institutes of Health (to G.E.P.). We would also like to acknowledge David Moore of Siena College, who assisted with the web site.